Coding

Part:BBa_K3806012:Design

Designed by: Laura Sierra Heras   Group: iGEM21_TUDelft   (2021-09-30)


lacZ reporter gene codon optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2193
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3806012 contains a codon optimized lacZ gene for optimal expression in E. coli. The codon optimization was performed with the Twist Codon Optimizer Tool.